Based upon protocols reccommended in the US Pharmacopoeia (USP 34), our mold assay utilizes two nutrient media to assess the prsence of fungi (molds and mildews) and bacteria. To determine the fungal load, a selective growth medium called Sabouraud's Dextrose Agar is employed. Because of its low pH, this medium inhibits the growth of most bacteria while allowing the growth of many fungi. Filamentous molds and mildews are easily detected on this medium. To determine the bacterial load of a sample, we innoculate a growth medium called Nutrient Agar, which supports the growth of many bacterial and yeast species. The upper limit of bacterial load is then determined by total counts of colony forming units with mucoid growth habit. All assays are performed in triplicate.